Isotype Antibody Controls
Isotype control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype matched control that has no specificity to any component of your testing materials provides some idea of the amount of non-specific binding that you may get with your specific antibody. In order to have a reasonable comparison, the isotype matched control should be the same species and isotype as your specific antibody, as well as at the same concentration. Isotype controls are only useful as a gross estimate of non-specific binding of your specific antibody.
